Legal. FOIA 2008 Sep;45(Pt 5):452-62. doi: 10.1258/acb.2008.008076. 4 DETERMINATION OF BLOOD GLUCOSE BY ENZYME METHODS 15 4.1 Glucose oxidase method 15 4.2 Hexokinase method 16 4.3 Glucose dehydrogenase method (GDH) 17 5 THE RESEARCH RESULTS 18 5.1 Research subjects 18 5.2 Survey results 24 6 CONCLUSIONS 26 REFERENCES 27 FIGURE 1: Glucose Transporter in Red Blood Cell. Glucose measurement - glucose oxidase The consumption of oxygen or production of hydrogen peroxide that results from glucose oxidase action allows amperometric measurement of glucose. Free resources to assist you with your university studies! We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. One centimetre cubed was inoculated into T1, 0.8 cm3 into T2, 0.6 cm3 into T3, 0.4 cm3 into T4 and 0.2 cm3 into T5. GOX catalyses the oxidation of d-glucose (C6H12O6) to d-gluconolactone (C6H10O6) and . 2005 Dec;42(6):391-4. advantages and disadvantages of glucose oxidase methodoregon dmv license renewal real id. Also, colonies taken from media containing high levels of glucose may give false-negative reactions. Its results are very reliable and specific, (Bauninger, 1974). A glucose standard curve is a method of monitoring blood glucose The higher the number, the more active the enzyme. [10], GOx is a dimeric protein, the 3D structure of which has been elucidated. One centimetre cubed of different sugars were inoculated in specific S tubes. Both methods employ a single premixed solution to perform the reaction, which may be purchased in kit form. Published by at February 16, 2022. Click the account icon in the top right to: Oxford Academic is home to a wide variety of products. Calculate the concentration of blood glucose in the specimen using the following formula: Your email address will not be published. For librarians and administrators, your personal account also provides access to institutional account management. You can see your glucose level anytime at a glance. Accurate and precise measurement of blood glucose level is of great importance in the diagnosis and management of diabetes and other disorders of carbohydrate metabolism, hyperglycemia, and hypoglycemia. To solve this question, the selection of biocompatible components is promising to improve the situation. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. To allow valid glucose determinations by the enzymic method, glutathione, cysteine, and amylase substrates such as glycogen must be absent. Oxidase positive bacteria possess cytochrome oxidase or indophenol oxidase (an iron containing haemoprotein). Fructose is abundant in fruits. The unknown glucoses were determined by the use of the graph. The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of $\beta$ -D- glucose to D-glucono- $\delta$ -lacto. The test tubes were marked T1 to T6, for those that had to be inoculated with glucose and S1 to S6, for those that had to be inoculated with different types of sugars. All members of the Enterobacteriaceae can . Direct Plate Method (7, 10) (Fig. If glucose is found in your urine it is called glycosuria or glucosuria. To eliminate interference of chloride with estimation of blood glucose, the samples must be diluted appropriately before analysis. In the GOx-catalyzed redox reaction, FAD works as the initial electron acceptor and is reduced to FADH. The FCC notation stands for Foods Chemical Codex and is a division of USP (United States Pharmacopeia). Any color changes appearing after 20 seconds should be disregarded. The common methods for the enzymatic deter mination of glucose involve several reaction steps, Linear output, low power requirements and good resolution. A strip of filter paper is soaked with a little freshly made 1% solution of the reagent. It might have the same compounds and properties as the stock solution. Glucose oxidase is measured in GO (glucose oxidase units), whereby one glucose oxidase unit is the amount of enzyme that will oxidize 1 mol of glucose per minute under the assay conditions. Mutat Res. Jose A. rodrigue - caqstelion et al, has reported a correlation value r = 0.98 between both the Into a series of test tube pipette out 0 (blank). HHS Vulnerability Disclosure, Help and transmitted securely. The Essay Writing ExpertsUK Essay Experts. Add 2 -3 drops of reagent directly to suspect colonies on an agar plate. as a screening test, the glucose oxidase method will not detect increased levels of galactose or other sugars in urine. 201, Block - B, Guru Gobind Singh Indraprastha University Made by: Nikita Gupta Enrollment no-01140801014 Mtech-NST (1st Sem) 2. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. J Pediatr Surg. Analyte: Plasma Glucose Matrix: Serum Method: Enzyme Hexokinase (HK) Method No. It is also simple straight forward and easy to manipulate. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. RELATED READING: Chapter 32. Then add 1mL of glucose oxidase-peroxidase reagent. We don't need all that extra energy. Download the App! Different methods based on different properties of glucose are described for blood glucose estimation. These include, but are not limited to, the filter paper test, direct plate method, swab method, impregnated oxidase test strip method and test tube method. Shibboleth / Open Athens technology is used to provide single sign-on between your institutions website and Oxford Academic. This reaction simultaneously consumes the glucose and exhibits significant activity against harmful organisms due to the production of hydrogen peroxide. Comparison of electrolyte and glucose levels measured by a blood gas analyzer and an automated biochemistry analyzer among hospitalized patients. All work is written to order. reading, the student will be able to: The Trinder method for glucose analysis utilizes the coupled enzyme reactions shown below: \[Glucose + O_{2} \xrightarrow{\text{glucose oxidase}} glucuronic\; acid + H_{2} O_{2}\], \[H_{2} O_{2} + reduced\; dye \xrightarrow{\text{horseradish peroxidase}} oxidized\; dye + H_{2} O\]. Incubate all the tube at 35C for 40 minutes. Please enable it to take advantage of the complete set of features! Redox reactions involve a gain or loss of electrons from a molecule. Consequently, a patient may become symptomatically hypoglycemic before a blood sample from one of these alternative sites shows a low glucose level. Shake vigorously to ensure mixing and thorough oxygenation of the culture. It is therefore important that a copper reduction . If you have a severe medical condition or health concern, see your physician. Observe for color change within 10 seconds. Careers. Record the appearance of the serum samples on the data sheet. 2021 Global Healing | All Rights Reserved, Up to 20% Off + FREE U.S. It is a point of high energy and requires more energy than the substrates. Glucose oxidase, also called notatin, is one of the many useful enzymes that nature has given us, and is produced by fungi, bacteria, and insects. Its efficiency depends on the concentration of the substrate and conditions like temperature or pH, (Hames et al, 2005). Calculate each glucose concentration using the absorbance and concentration of the standard by the proportion method (see Exercise #1). Microorganisms are delayed oxidase positive when the color changes to purple within 2 to 3 minutes. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. When using Kovacs oxidase reagent, microorganisms are oxidase positive when the color changes to dark purple within 5 to 10 seconds. INTRODUCTION Tolazamide has been shown to act as a competitive oxygen acceptor1. To allow valid glucose determinations by the enzymic method, glutathione, cysteine, and amylase substrates such as glycogen must be absent. because of its specificity. Do not use an Oxford Academic personal account. . We constructed the user-defined endpoint methods on three automated analyzers. difference between fact and truth. Educator app for It undergoes a series of chemical reactions to produce energy. These products are not intended to diagnose, treat, cure, or prevent any disease. Label sufficient 13 x 100 mm test tubes for each standard, control, and patient sample to be tested. Collect fresh urine specimen in a clean dry container with labeling. Its structure consists of six carbons with a glycosidic bond to join the next glucose molecule, (Berg et al, 2007). The evolution of 1st to 3rd generation electrochemical biosensors reflects a simplification and enhancement of the transduction pathway. . In order to work as a catalyst, GOx requires a coenzyme, flavin adenine dinucleotide (FAD). The .gov means its official. Plasma Glial Fibrillary Acidic Protein in the Alzheimer Disease Continuum: Relationship to Other Biomarkers, Differential Diagnosis, and Prediction of Clinical Progression, Commentary on Unexpected Fentanyl Detection during a Liver Transplant Evaluation, Postoperative Drainage Fluid with a Syrupy Appearance, Increasing Parechovirus Central Nervous System Infections and the Enduring Value of Clinical Microbiology Laboratories, Clinical Chemistry Guide to Scientific Writing, Clinical Chemistry Guide to Manuscript Review, https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model, Subscription prices and ordering for this journal, Purchasing options for books and journals across Oxford Academic, Receive exclusive offers and updates from Oxford Academic. 0.2, 0.4, 0.6, 0.8 and 1mL of working standard glucose solution and make up the volume to 1.0mL with distilled water. (vi) Folic acid is a molecule used by ALL cells for growth. A speck of culture is rubbed on it with a platinum loop. Clinical Chemistry - Theory, Analysis, Correlation (Kaplan and Pesce), { "2.01:_Basic_Spectrophotometry" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.
b__1]()", "2.02:_Absorbance_Spectra" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.03:_Standard_Curve" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.04:_Buffer_Preparation" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.05:_Buffer_Titration_buffering_Capacity" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.06:_Flame_Photometer" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.07:_Chloride_Determination_(Coulometric_Method)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.08:_Electrolytes_anion_Gap" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.09:_Serum_Osmolality" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.10:_Serum_Glucose-_Trinder_Method" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.11:_Glucose_Tolerance_Test" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.12:_Total_Serum_Protein" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.13:_Serum_Albumin_(Bromcresol_Green_Method)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.14:_Serum_Creatinine_(Jaffe_Method)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.15:_Bilirubin_(Waters_and_Gerande__DMSO_Method)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.16:_Cholesterol_(Total_and_HDL)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.17:_Triglycerides" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.18:_Lipoprotein_Electrophoresis" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.19:_Kinetic_Enzyme_Analysis" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.20:_Enzymes_Rate_Creatine_Kinase_(CK)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.21:_Amylase_(Modified_Caraway_Method)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.22:_Creatine_Kinase_(CK)_Isoenzyme_Electrophoresis" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.23:_Blood_Gas_Analysis" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.24:_Phlebotomy" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.25:_Exercise_25_Quality_Control" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.26:_Extraction_Technique" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.27:_Thin_Layer_Chromatography_(TLC)_of_Drugs" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.28:_High_Performance_Liquid_Chromatography_(HPLC)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.29:_Gas_Liquid_Chromatography_(GLC)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2.30:_Radioimmunoassay_(RIA)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "01:_Questions_and_Answers" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "02:_Laboratory_Exercises" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "03:_Urinalysis" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "04:_Case_Histories" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "zz:_Back_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "showtoc:no", "license:ccbyncsa", "licenseversion:40" ], https://med.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fmed.libretexts.org%2FBookshelves%2FAllied_Health%2FBook%253A_Clinical_Chemistry_-_Theory_Analysis_Correlation_(Kaplan_and_Pesce)%2F02%253A_Laboratory_Exercises%2F2.10%253A_Serum_Glucose-_Trinder_Method, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), status page at https://status.libretexts.org. Glucose oxidase catalyzes a reaction in which glucose sugar is broken down into hydrogen peroxide and gluconolactone. This method was first described by Trinder in 1969 thus named after him, (Lott et al, 1975). When it takes more than 2 minutes to colour change, it may the reason of some contaminated microbes from the air between the 2 minutes, Could we perform the oxidase test from colonies taken directly from selective media such as Chromocult Coliform agar, CCA? Product reviews are within range of typicality. When a drop of blood is placed on the strip, the . Factors, affecting these assays are discussed: specificity of the enzymes used (cholesterol esterase and cholesterol oxidase), conditions of analysis and composition of the reaction mixture, interference with components of blood serum. Is this reaction more or less specific than a glucose oxidase/oxygen electrode method? Publication types Evaluation Study Oxidase Negative Organisms: Enterobacteriaceae (e.g. This method gives much lower blood glucose values with blood samples of neonatal jaundice (plasma bilirubin level > 10 mg/dL) of age 10 +/- 5 daysthan with samples of neonates of the same age group without jaundice or older children suffering from other diseases like acute respiratory distress, septicemia. Also, colonies taken from media containing high levels of glucose may give false-negative reactions. Give the main points of differences between glucose and fructose. glucose estimation even in a comlpex mixture. An equation was used also to determine the concentrations. Glucose-6-phosphate dehydrogenase unit definition: One unit will oxidize 1.0 mol of D-glucose 6-phosphate to 6-phospho-D-gluconate per minute in the presence of -NADP at pH 7.4 at 25 C. Oxidase Positive Organisms: Pseudomonas, Neisseria, Alcaligens, Aeromonas, Campylobacter, Vibrio, Brucella, Pasteurella, Moraxella, Helicobacter pylori, Legionella pneumophila, etc. iPad. Microorganisms are delayed oxidase positive when the color changes to purple within 60 to 90 seconds. One limitation to mercury as a working electrode is the ease with which it is oxidized. pH, Specific Gravity, Glucose, Protein, Ketones, Urobilinogen, Blood, Bilirubin. What are the major advantages and disadvantages of the glucose oxidase/peroxidase method of determining glucose? Apart from these advantages, however, glucose limitation provoked phenomena with profound effect on cell physiology and process efficiency. The oxidase test detects the presence of a cytochrome oxidase system that will catalyse the transport of electrons between electron donors in the bacteria and a redox dye- tetramethyl-p-phenylene-diamine. And glucose oxidase also uses oxygen as an external electronic sector that releases H 202. It prevent fading. In the case of chemical assays, this term refers to the point when the reaction is essentially completed and there is no net production of reaction product. However, though this simple test is known since long time, it is ambiguous in the interpretation of results and gives wrong information required in bacterial identification. The line of best fit was used to determine the glucose concentration. It is advantageous compared to the pure culture method The same pipette might have been used to transfer the solutions thus cross contamination. allows second-chance sampling, meaning you can apply more blood to the test strip . Ingested glucose is absorbed directly into the blood from the intestine and results in a rapid increase in blood glucose. organisms. Both bacteria and yeast grown on media containing high concentrations of glucose show inhibited oxidase activity, so it is recommended to test colonies grown on media without excess sugar, such as nutrient agar. This method gives much lower blood glucose values with blood samples of neonatal jaundice (plasma bilirubin level > 10 mg/dL) of age 10 +/- 5 daysthan with samples of neonates of the same age group without jaundice or older children suffering from other . Do not use an Oxford Academic personal account. These different absorbencies were recorded on a table. *You can also browse our support articles here >. View your signed in personal account and access account management features. These include, but are not limited to, the filter paper test, direct plate method, swab method, impregnated oxidase test strip method and test tube method. Without release, the duration of antibacterial activity may be significantly longer. The anthrone reaction with glucose is enhanced by chloride, bromide, and iodide. glucose oxidase and Bethesda, MD 20894, Web Policies Using appropriate cuvettes, measure the absorbance of each reaction mixture at 525 nm against a water blank. FAD is a common component in biological oxidation-reduction (redox) reactions. Human cells do not have an enzyme to make PABA. The Trinder method is used to determine glucose concentration only, (Lott et al, 1975). It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. 1. i think Haemophilus is regarded as a late oxydase positive organism i.e positive 30 to 60 seconds (as opposed to immediate positve organisms), usually we use Escherichia coli ATCC as a negative control on abrerant late positive results, Hello please is Borrelia a positive oxidase or negative? It is mostly abundant in the nucleotides. When on the society site, please use the credentials provided by that society. ADVANTAGES : f Hexokinase method PRINCIPLE: Glucose +ATPGlucose 6 phosphate +ADP Glucose 6 Phosphate + NAD 6- Phosphogluconate + NADH+H Conversion of NADH from NAD at 340nm ,increase in O.D. Describe the principle of the glucose oxidase method of reagent strip testing for glucose, and name possible causes of interference with this method . Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more. Measure the absorbance of the standard and test sample at 540nm (green filter) against blank within 60 minutes. Similar assays allows the monitoring of glucose levels in fermentation, bioreactors, and to control glucose in vegetal raw material and food products. compatible with all Contour Next glucose meter models. [15] It is also used as a food preservative to help remove oxygen and glucose from food when packaged such as dry egg powder to prevent unwanted browning and undesired taste.[16]. Grow a fresh culture (18 to 24 hours) of bacteria in 4.5 ml of nutrient broth (or standard media that does not contain a high concentration of sugar). Glucose oxidase offers multiple health benefits including digestive support, blood sugar normalization, and more. Flavinadenine dinucleotide (FAD) in the active site is buried approximately 1.5 nm inside the protein shell and acts as the initial electron acceptor. It doesn't target other sugars except glucose. The most widely used method for estimation of plasma glucose is that adopted by Trinder's using glucose oxidase-peroxidase (GOD-POD) system. Additionally, the Reviewed and Approved seal signifies that our scientific board of experts has double-checked this article for accuracy. Epub 2009 Apr 17. When the enzyme was applied to the other sugars, there was no absorbance at all because the enzyme is specific to one substrate thus it didnt catalyse the reaction of other sugars and GODPOD. Terminate the reaction by the addition of 2mL of 6 N-HCl. 0.5mM of Glucose was transferred using a P100 pipette and not changing the tip. Any scientific information contained within this essay should not be treated as fact, this content is to be used for educational purposes only and may contain factual inaccuracies or be out of date. Enter your library card number to sign in. It also is helpful in isolating In this study, GOD activity determination methods were reviewed mainly four approaches: determination of decrease in glucose or oxygen concentration and determination of increase in hydrogen. What is the metabolic advantage in the conversion of glucose to lactate, in which there is no net oxidation or reduction? Get 24/7 study help with the Numerade app for iOS and Android! We are not responsible for the content or products of any linked site or any link contained in a linked site. Pipet 2.0 mL of glucose reagent into each tube. ( Im using oxidase strip ). Such links are provided for your convenience and reference only. [ 7 ] The overall effect of this is that the blood leaving the machine and returning into the patient . First, CGM avoids many inconveniences such as physical and psychological pain that are normally associated with the standard method of capillary blood glucose testing.